Maurizio Fraziano    

Curriculum Vitae
(1992)Degree in Biological Sciences, at the University of Rome “La Sapienza”; (1996) PhD in “Cellular Differentiation and Morphogenesis” at the University of Rome “Tor Vergata; (1995) PhD student at the Division of Oncology of “St. George’s Hospital Medical School”, London (Scientific Director: Prof. A.G. Dalgleish); (1996-2001) UNESCO fellowship at the Department of Biology, University of Rome “Tor Vergata”; (2001 to present) Researcher atthe Department of Biology, University of Rome “Tor Vergata”. He is author of 43 scientific papers on indexed journals.

Teaching activity
(2001 to now): Immunotechnology (2 ECTS), Immunology (3 ECTS), Molecular basis of cell response to infections (3 ECTS), Immunology and Pathology (6 ECTS), Applied Immunology (6 ECTS).

Patents
Fraziano M., Garg S.K., Volpe E., Martino A., De Vito P., Baldini M., Galati D., Auricchio G., Ciaramella A., Colizzi V. Immunoregulatory compounds. Brevetto No. PCT/IT02/00740.
Fraziano M., De Spirito M., Greco E., Quintiliani G. Liposomiasimmetrici e lorousi in campo medico.Brevetto No. RM2007A000394 del 16 luglio 2007.

Research topics
• Host pathogen interaction in the field of Mycobacterium tuberculosis infection: analysis of the role played by second lipid messengers in the activation of i) antimycobacterialinnate immune response, ii) autophagy, and iii) phagolysosome maturation.
• Identification of novel adjuvants: analysis of endogenous adjuvants for the generation of novel anti-tuberculosis vaccine formulations.
• Identification of novel immunotherapeutic anti-infectious strategies: generation of novel asymmetric apoptotic body-like liposomes and their analysis in terms of capability to modulate antimicrobial and inflammatory response in vitro, in vivo (in murine models) and ex vivo (in cells from cystic fibrosis patients) experimental settings.

Fundings (last 5 years)
MIUR – PRIN 2009 (24.000 euro).
Ministerodella Salute – Bando AIDS 2009 (97.920 euro)
FondazioneItalianaFibrosiCistica 2013 (50.000 euro).

Active collaborations (last 2 years)
Prof. M. Sanguinetti e Prof. G. Delogu, Catholic University of Sacred Heart “A. Gemelli”, Roma; Prof. F. Dieli, University of Palermo; Dr. R. Nisini, ISS, Roma; Dr. D. Goletti; INMI L. Spallanzani, Roma; Prof. T.H.M. Ottenhoff, Leiden University Medical Center (The Netherland); Dr. R. Reljic, S. George’s University of London (UK).

Publications(last 5years)
1. Sali M., Delogu G., Greco E., Rocca S., Colizzi V., Fadda G.,Fraziano M. Exploiting immunotherapy in Mycobacterium tuberculosis-infected mice:Sphingosine 1-phosphate treatment results in a protective or detrimental effect depending by the stage of infection (Int. J. Immunopathol. Pharmacol., 2009; 22: 175-81)
2. MaulucciG. PaniG., FuscoS., PapiM., ArcovitoG., GaleottiT., Fraziano M., De SpiritoM. Compartmentalization of the redox environment in PC-12 neuronal cells. (Eur. Biophys. J., 2010;39:993-9)
3. Greco E., Santucci M.B., Papi M., De Spirito M, Fraziano M.CpG oligodeoxynucleotides promote endolysosome maturation and intracellularmycobacterial killing in M. tuberculosis infected type II alveolar epithelial cells (Cell Immunol,2009; 259: 1-4)
4. Greco E., Sali M., De AngelisRigotti F., Papi M., De Spirito M.,Delogu G., Fraziano M.Natural lysophospholipids reduce Mycobacterium tuberculosis-induced cytotoxicity and induce anti-mycobacterial activity by a phagolysosome maturation-dependent mechanism in A549 type II alveolar epithelial cells. (Immunology,2010; 129: 125-32)
5. Ouattara L., Koudou J., Karou D.S., Giacò L., Cappelli G., Simpore J., Fraziano M., Colizzi V., Traore A.S. In vitro anti Mycobacterium tuberculosis H37Rv activity of Lannea acida A. Rich from Burkina Faso.(Pak J Biol Sci. 2011; 14: 47-52)
6. Delogu G., Sali M., Rocca S., Quintiliani G., Santucci M.B., Greco E., Cabibbo A., Mariani F., Colizzi V., Fadda G., Fraziano M. Lysophosphatidic acid enhances antimycobacterial response during in vivo primari Mycobacterium tuberculosis infection(Cell Immunol. 2011; 271: 1-4)
7. Papi M, Palmieri V., Maulucci G., Arcovito G., Greco E., Quintiliani G., Fraziano M., De Spirito M. Controlled self assembly of colagen nanoparticle. (J Nanopart Res 2011; 13:6141–6147)
8. Iantomasi R., Sali M., Cascioferro A., Palucci I., Zumbo A., Soldini S., RoccaS., Greco E., Maulucci G., De Spirito M., Fraziano M., Fadda G., Manganelli R., Delogu G. PE_PGRS30 is required for the full virulence of Mycobacterium tuberculosis. (Cell Microbiol. 2012; 14: 356-67)
9. Giacò L., Amicosante M., Fraziano M., Gherardini P.F., Ausiello G., Helmer-Citterich M., Colizzi V., Cabibbo A. B-Pred, a structure based B-cell epitopes prediction server.(Adv Appl Bioinforma Chem,2012; 5: 11-21)
10. Giovannini D., Cappelli G., Jiang L., Castilletti C., Colone A., Serafino A., Wannenes F., Giacò L., Quintiliani G., Fraziano M., Nepravishta R., Colizzi V., Mariani F.A new Mycobacterium tuberculosis smooth colony reduces growth 1 inside human macrophages and represses PDIM Operon gene expression. Does an heterogeneous population exist in intracellular mycobacteria? (Microb pathogen, 2012; 53: 135-46).
11. Greco E, Quintiliani G, Santucci MB, Serafino A, Ciccaglione AR, Marcantonio C, Papi M, Maulucci G, Delogu G, Martino A, Goletti D, Sarmati L, Andreoni M, Altieri A, Alma M, Caccamo N, Di Liberto D, De Spirito M, Savage ND, Nisini R, Dieli F, Ottenhoff TH, Fraziano M. Janus-faced-liposomes enhance anti-microbial innate immune response in Mycobacterium tuberculosis infection (Proc Natl Acad Sci USA, 2012; 109: E1360-8).
This manuscript has been commented on:
Sci Signal 2012, Vol. 5, Iissue N. 226 5, p. ec149, Nancy R. Gough, Fighting infection with liposomeshttp://stke.sciencemag.org/cgi/content/abstract/sigtrans;5/226/ec149
12. Mariotti S., Sargentini V., Pardini M., Giannoni F., De Spirito M., Gagliardi M.C., Greco E., Teloni R., Fraziano M., Nisini R. Mycobacterium tuberculosis may escape helper T cell recognition by infecting human fibroblasts(Hum Immunol, 2013; 74:722-9)
13. Mariotti S., Pardini M., Gagliardi MC, Teloni R, Giannoni F, Lozupone F, Fraziano M., Meschini S, Nisini R. Dormant Mycobacterium tuberculosis Fails to Block Phagosome Maturation and Shows Unexpected Capacity to Stimulate Specific Human T Lymphocytes (J. Immunol., 2013; 191:274-82)